Direct Diagnosis of Helicobacter pylori Infection by Detection of specific IgG antibodies in saliva of Iraqi patients

Detection of helicobacter pylori (H. pylori) by invasive methods is costy and unpleasant for patients and is not free form complication ,thus in our study we evaluated an enzyme linked immunosorbent assay (ELISA) for measurement of H. pylori specific IgG in saliva in comparison with biopsy urease test and serum IgG.from 100 patients consult gastrointestinal in Najaf Hospitals during the period from February 2006 to March 2007 detailed history was taken and blood group was detected for each one .then the patients underwent endoscopy and serum and saliva was collected from each one. urease was done for samples collected by endoscopy . serum and saliva was evaluated for presence of specific IgG by ELISA kit. Our results showed that 42(82%) of urease positive patients had IgG in saliva, whereas 47(94%) urease positive patients had IgG in serum and no statistical differences (p-value >0.1).the result show highest concentration of saliva IgG in urease positive patients. There is significant difference between saliva specific IgG for H.pylori with age groups 31-40 years, drink Pepsi more than 4 cup/day, patients who are smoker and those with blood group O. we conclude that salivary IgG detected by ELISA is a rapid ,easy and accurate method for detection and screening for H.pylori among different age groups.